Journal: Journal of Xenobiotics
Article Title: Chronic Exposure to Both Electronic and Conventional Cigarettes Alters Ileum and Colon Turnover, Immune Function, and Barrier Integrity in Mice
doi: 10.3390/jox14030053
Figure Lengend Snippet: Effects of chronic electronic and conventional cigarette exposure on ileum and colon proliferation. Ki-67 immunostaining and quantification of Ki-67-positive cells per crypt in ileum ( A ) and colon ( B ). Ki67 stain: green. Nuclear stain: blue. n = 6 per group. ** p < 0.01 compared to the control group (Air), as determined by the Mann–Whitney U test. Scale bar = 50 µm.
Article Snippet: Rabbit anti-mouse Ki-67 primary antibody (MA5-14520, Invitrogen; Fisher Scientific, Illkirch, France 1/100 dilution), rabbit anti-mouse cyclin D1 primary antibody (#55506, Cell Signaling Technology; Danvers, USA 1/400 dilution), rabbit anti-mouse ZO-1 primary antibody (#61-7300, Invitrogen; 1/200 dilution), and rabbit anti-mouse occludin primary antibody (#91131, Cell Signaling; 1/200 dilution) were incubated overnight at 4 °C.
Techniques: Immunostaining, Staining, Control, MANN-WHITNEY